Dual labelled probes - DLPs

 

Our Standard Dual-labelled fluorogenic probes Portfolio

 

5 yield ranges* based on length independent delivered quantities from 6-40 bases.

  • * ≥ 5 < 10 nmol
  • * ≥ 10 < 20 nmol
  • * ≥ 20 < 30 nmol
  • * ≥ 30 < 50 nmol
  • * ≥ 50 < 70 nmol

** based on oligonucleotides of 6-40 bases in length; please note that HPLC purification and QC by Mass-Check is included

 

Reporter-Quencher combinations

3' Quencher
  TAMRA BHQ®-1 BHQ®-2 BBQ®-650 BHQ®-3 Eclipse® Dabcyl    
Abs range (nm) 470-560 480-580 550-650 550-750 620-730 390-625 380-550    
Abs max (nm) 544 535 579 650 672 522 453    
5' Reporter Possible combinations Abs (nm) Em (nm)
Atto 390 O         O O 390 476
Atto 425 O O       O O 439 485
LC® CYAN500   O       O O 450 500
6-Fam O O O     O O 495 520
Fluo O O O     O O 495 520
FITC O O O     O O 490 525
Atto 495  O O       O O 498 526
Tet O O O     O O 521 536
Atto 520  O O       O O 517 538
Joe O O       O O 522 548
Yakima Yellow® O O       O O 530 549
Hex O O O O   O O 535 556
Atto Rho6G  O O O O   O O 533 557
Cy3     O O   O   546 563
Tamra     O O   O O 564 579
Rox O   O O   O   576 601
Texas Red®     O O   O   586 610
LC® Red 610     O O   O   590 610
Atto Rho13      O O O     603 627
DY480XL     O O       500 630
LC® Red 640     O O O     625 640
Atto Rho14      O O O     625 646
Cy5     O O O     646 662
Cy5.5 O   O O O     683 705
IRD®700 O   O O O     685 705

 

5' Quencher
  BHQ®-1 BHQ®-2 BBQ®-650 Eclipse® Dabcyl    
Abs range (nm) 480-580 550-650 550-750 390-625 380-550    
Abs max (nm) 535 579 650 522 453    
3' Reporter Possible combinations Abs (nm) Em (nm)
6-Fam O O   O O 495 520
Fluo O O   O O 495 520
Joe O     O O 522 548
Hex O O   O   535 556
Cy3   O       546 563
Rox   O O O   576 601
LC® Red 610   O O O   590 610
LC® Red 640   O O     625 640
Cy5   O O     646 662
Cy5.5   O O     683 705

All our DLP probes are HPLC purified and mass-checked for best quality assurance!

>HPLC purification and QC by Mass-Check is included

 

Reporter-Quencher combinations

3' Quencher
  TAMRA BHQ®-1 BHQ®-2 BBQ®-650 BHQ®-3 Eclipse® Dabcyl    
Abs range (nm) 470-560 480-580 550-650 550-750 620-730 390-625 380-550    
Abs max (nm) 544 535 579 650 672 522 453    
5' Reporter Possible combinations Abs (nm) Em (nm)
Atto 390 O         O O 390 476
Atto 425 O O       O O 439 485
LC® CYAN500   O       O O 450 500
6-Fam O O O     O O 495 520
Fluo O O O     O O 495 520
FITC O O O     O O 490 525
Atto 495  O O       O O 498 526
Tet O O O     O O 521 536
Atto 520  O O       O O 517 538
Joe O O       O O 522 548
Yakima Yellow® O O       O O 530 549
Hex O O O O   O O 535 556
Atto Rho6G  O O O O   O O 533 557
Cy3     O O   O   546 563
Tamra     O O   O O 564 579
Rox O   O O   O   576 601
Texas Red®     O O   O   586 610
LC® Red 610     O O   O   590 610
Atto Rho13      O O O     603 627
DY480XL     O O       500 630
LC® Red 640     O O O     625 640
Atto Rho14      O O O     625 646
Cy5     O O O     646 662
Cy5.5 O   O O O     683 705
IRD®700 O   O O O     685 705

 

5' Quencher
  BHQ®-1 BHQ®-2 BBQ®-650 Eclipse® Dabcyl    
Abs range (nm) 480-580 550-650 550-750 390-625 380-550    
Abs max (nm) 535 579 650 522 453    
3' Reporter Possible combinations Abs (nm) Em (nm)
6-Fam O O   O O 495 520
Fluo O O   O O 495 520
Joe O     O O 522 548
Hex O O   O   535 556
Cy3   O       546 563
Rox   O O O   576 601
LC® Red 610   O O O   590 610
LC® Red 640   O O     625 640
Cy5   O O     646 662
Cy5.5   O O     683 705

All our DLP probes are HPLC purified and mass-checked for best quality assurance!