dsDNA
Our duplex DNA service includes
- O synthesis, purification and QC of 2 complementary DNA oligonucleotides up to a length of 220 nts.
- O equimolar mixing of individually QCed DNA oligos (analytical HPLC and Mass Check).
- O annealing of the two oligos, formation of the respective duplex DNA, and "freezing" of the ds-status as dried down deliverable.
- O QC of DNA duplex by ESI-ToF mass spectrometry.
- O Providing recommendations/ a protocol to re-dilute, re-anneal and use the delivered dsDNA oligos.
A full list of standard modification is available below:
Fluorencent Modifications
| Type | Position | Spectrum Maxima | |||
|---|---|---|---|---|---|
| 5' | 3' | internal | Abs | Em | |
| Atto425 | O | O | O | 439 | 485 |
| 6-Fam | O | O | O | 495 | 520 |
| Fluo | O | O | O | 495 | 520 |
| FITC | O | O | O | 490 | 525 |
| Tet | O | O | O | 521 | 536 |
| Joe | O | O | O | 522 | 548 |
| Yakima Yellow® | O | O | 530 | 549 | |
| Hex | O | O | O | 535 | 556 |
| Cy3 | O | O | O | 546 | 563 |
| Atto550 | O | O | O | 554 | 576 |
| Tamra | O | O | O | 564 | 579 |
| Rox | O | O | O | 576 | 601 |
| Texas Red® | O | O | O | 586 | 610 |
| Cy5 | O | O | O | 646 | 662 |
| Atto647N | O | O | 646 | 664 | |
| Cy5.5 | O | O | O | 683 | 705 |
| IRD®700 | O | O | 685 | 705 | |
| IRD®800 | O | O | 787 | 812 | |
Concentrations available: 0,5 to 200 nmol
Purification available: HPLC
Non-Fluorescent Modifications
| Type | Position | ||
|---|---|---|---|
| 5' | 3' | internal | |
| Phosphate | O | O | |
| C6 Amino | O | ||
| C12 Amino | O | ||
| Biotin | O | O | O |
| C6 Thiol | O | O | |
| Digoxigenin | O | O | O |
| Inosine | O | O | O |
| 2' Deoxyuridine | O | O | O |
| 5-Me-dC | O | O | O |
| Alxyl | O | ||
| AldC | O | O | O |
| AldU | O | O | O |
| C3 spacer | O | O | O |
| C9 spacer | O | O | |
| HEG spacer | O | O | O |
| Cholesteryl-TEG | O | O | |
| C7 Amino | O | ||
| ddC | O | ||
| Inverted T | O | ||
| Dabcyl | O | ||
| BHQ®-1 | O | ||
| BHQ®-2 | O | ||
| Alrol | O | ||
| C2-Amino dT | O | ||
| C6-Amino dT | O | ||
| dspacer | O | ||
| pdU | O | ||
| C6 spacer | O | ||
| C12 spacer | O | ||
| C3 Thiol | O | ||
