(주)바이오디 - Biosis Design


ZNA primers and probes

 

What we offer

 

  • O Primers as short as 10mers and dual labelled probes as short as 8mers, because the relative Tm increasing effect of ZNA (or other duplex stabilizing groups) is naturally higher on shorter oligonucleotides used as primers or probes.
  • O The possibility to add ZNA-building blocks, both at the 3’ and at the 5’-end of your DNA sequence.
  • O Standard length range between 10 and 40 nucleotides for primers and probes. For longer sequences, please inquire.
  • O For primers4 standard ZNA building block modifications: ZNA-2, ZNA-3, ZNA-4, and ZNA-5!

    • > ZNA-2 for oligos ≥ 8mers
    • > ZNA-3 for oligos ≥ 12mers
    • > ZNA-4 for oligos ≥ 16mers
    • > ZNA-5 for oligos ≥ 20mers
  • O For probes3 standard ZNA-Quencher (Q*ZNA-X) building block modifications for 3´labeling (For 5´ ZNA-Reporter labeling, please inquire!); Respective choice of 3´building blocks will be made automatically by metabion according to the following scheme:

    • > 3' Q*ZNA-2 for probes ≥ 8mers
    • > 3' Q*ZNA-3 for probes ≥ 14mers
    • > 3' Q*ZNA-4 for probes ≥ 18mers
  • O Additional modifications, like C-5 propynyl-dC (pdC) and C-5 propynyl-dU (pdU), which will further raise the Tm to ~2.8°C and ~1.7°C per substitution, respectively.
  • O HPLC and documented Mass-Check QC included
  • O 5´ZNA modified dual labeled probes, introducing 5´reporters like FAM, Fluo, JOE, ROX, TAMRA, etc (see table below)

With appropriate design, ZNA probes can be used for high resolution post-PCR melting curve analysis (HRM). Please inquire at info@metabion.com and we will prepare a design for you!

All ZNA® oligos will be delivered dissolved in purified distilled water (pH 7-9), with a concentration of 100 µM (for special requests, please inquire)

Reporter-quencher combination

3' Quencher
  BHQ®-1 BHQ®-2 BBQ®-650 Eclipse® Dabcyl    
Abs range (nm) 480-580 550-650 550-750 390-625 380-550    
Abs max (nm) 535 579 650 522 453    
5' Reporter Possible combinations Abs (nm)* Em (nm)*
Fluo O     O O 450 500
6-FAM O     O O 495 520
TET O     O O 521 536
JOE O     O O 522 548
Yakima Yellow® O     O O 530 549
Hex O O O O O 535 556
Cy3   O O     546 563
ROX O O O O   576 601
Texas Red®   O O O   586 610
LC® Red 610   O O O   590 610
LC® Red 640   O O     625 640
Cy5   O O     646 662
Cy5.5   O O     683 705
IRD®700   O O     685 705

*Please note that absorption and emission wavelengths are influenced by the solvent (pH, salt conditions, etc.), oligo sequence and other factors.

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