ZNA primers and probes
What we offer
- O Primers as short as 10mers and dual labelled probes as short as 8mers, because the relative Tm increasing effect of ZNA (or other duplex stabilizing groups) is naturally higher on shorter oligonucleotides used as primers or probes.
- O The possibility to add ZNA-building blocks, both at the 3’ and at the 5’-end of your DNA sequence.
- O Standard length range between 10 and 40 nucleotides for primers and probes. For longer sequences, please inquire.
- O For primers, 4 standard ZNA building block modifications: ZNA-2, ZNA-3, ZNA-4, and ZNA-5!
- > ZNA-2 for oligos ≥ 8mers
- > ZNA-3 for oligos ≥ 12mers
- > ZNA-4 for oligos ≥ 16mers
- > ZNA-5 for oligos ≥ 20mers
- O For probes, 3 standard ZNA-Quencher (Q*ZNA-X) building block modifications for 3´labeling (For 5´ ZNA-Reporter labeling, please inquire!); Respective choice of 3´building blocks will be made automatically by metabion according to the following scheme:
- > 3' Q*ZNA-2 for probes ≥ 8mers
- > 3' Q*ZNA-3 for probes ≥ 14mers
- > 3' Q*ZNA-4 for probes ≥ 18mers
- O Additional modifications, like C-5 propynyl-dC (pdC) and C-5 propynyl-dU (pdU), which will further raise the Tm to ~2.8°C and ~1.7°C per substitution, respectively.
- O HPLC and documented Mass-Check QC included
- O 5´ZNA modified dual labeled probes, introducing 5´reporters like FAM, Fluo, JOE, ROX, TAMRA, etc (see table below)
With appropriate design, ZNA probes can be used for high resolution post-PCR melting curve analysis (HRM). Please inquire at info@metabion.com and we will prepare a design for you!
All ZNA® oligos will be delivered dissolved in purified distilled water (pH 7-9), with a concentration of 100 µM (for special requests, please inquire)
Reporter-quencher combination
3' Quencher | |||||||
---|---|---|---|---|---|---|---|
BHQ®-1 | BHQ®-2 | BBQ®-650 | Eclipse® | Dabcyl | |||
Abs range (nm) | 480-580 | 550-650 | 550-750 | 390-625 | 380-550 | ||
Abs max (nm) | 535 | 579 | 650 | 522 | 453 | ||
5' Reporter | Possible combinations | Abs (nm)* | Em (nm)* | ||||
Fluo | O | O | O | 450 | 500 | ||
6-FAM | O | O | O | 495 | 520 | ||
TET | O | O | O | 521 | 536 | ||
JOE | O | O | O | 522 | 548 | ||
Yakima Yellow® | O | O | O | 530 | 549 | ||
Hex | O | O | O | O | O | 535 | 556 |
Cy3 | O | O | 546 | 563 | |||
ROX | O | O | O | O | 576 | 601 | |
Texas Red® | O | O | O | 586 | 610 | ||
LC® Red 610 | O | O | O | 590 | 610 | ||
LC® Red 640 | O | O | 625 | 640 | |||
Cy5 | O | O | 646 | 662 | |||
Cy5.5 | O | O | 683 | 705 | |||
IRD®700 | O | O | 685 | 705 |
*Please note that absorption and emission wavelengths are influenced by the solvent (pH, salt conditions, etc.), oligo sequence and other factors.