CRISPRevolution Synthetic sgRNA Kit


Achieve up to 97% editing efficiency with the highest quality synthetic sgRNA in the market. Synthego is the first company to deliver a production scale, full length 100-mer synthetic sgRNA product at a practical price and volume.

Unlike custom developed solutions that are expensive and can take weeks before transfection readiness, CRISPRevolution sgRNA ships up to 4X faster and 80% lower cost.


Benefits of Synthetic sgRNA


No need to anneal crRNA:tracrRNA
Higher efficiency and reduced lab time


Better in vivo stability
When duplexed with Cas9 nuclease


Highly scalable for large numbers of experiments


100% DNA-free
No risk of integrating foreign DNA into cell line


A single oligonucleotide
Ready for transfection out-of-the box


Optimized for SpCas9
Optimized 80mer Synthego scaffold for use with SpCas9



No need to anneal, the sgRNA format arrives ready for transfection. It uses a single strand of user-defined 17-23nt RNA target sequence that will bind to the DNA target sequence that is opposite to the PAM sequence.


An optimized 80mer Synthego Scaffold is added on the 3′ end of your target sequence. For example, you will receive a sgRNA with the following format:

5′-AAUUUCACAGCUGCACAUA+Synthego Scaffold-3′



CRISPRevolution sgRNA Purity vs. IVT


Synthego Synthetic sgRNA


Superior quality leads to consistent editing efficiencies.



Mass-spec trace of synthetic AAVS1 gene 100nt sgRNA (Synthego). 

Large single peak represents full-length product.



IVT-derived Guide RNA


Increased impurities cause off-target effects.



Mass-spec trace of IVT-derived AAVS1 gene 105nt sgRNA.

Large n-1, n+1 and n+2 species are present.



CRISPRevolution sgRNA Efficiency & Consistency


The chart below compares the editing efficiency (solid bars) and consistency (black lines) of sgRNAs against in vitro transcribed guide RNAs in HEK293T cells.

The experiment was conducted by a third-party using three different gene targets and was replicated three times.




Working with Stem Cells or Other Difficult TargetsO Try Chemically Modified Synthetic RNA.


Chemically modified sequences can provide additional RNA stability with improvements in editing efficiency for particular cell types (e.g. stem cells, K562, prokaryotic) and genomic targets that prove otherwise challenging to edit. Please visit our Chemically Modified RNA page for more information.

For a chemically modified version of our synthetic RNA, we offer 2’-O-methyl analogs and 3’ phosphorothioate internucleotide linkages at the first three 5’ and 3’ terminal RNA residues. If you’re interested in a chemically modified option, select “Yes” to “Modified” from the dropdown menu when placing your order. Any questions, please contact us.

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